Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence ® Control siRNA (Unconjugated) #6568 (-), SignalSilence ® γ-Catenin siRNA I #6226 (+) or SignalSilence ® γ-Catenin siRNA II (+), using γ-Catenin Antibody #2309 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The γ-Catenin Antibody confirms silencing of γ-catenin expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence ® Control siRNA (Unconjugated) #6568 (-), SignalSilence ® γ-Catenin siRNA I #6226 (+) or SignalSilence ® γ-Catenin siRNA II (+), using β-Catenin Antibody #9562 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The β-Catenin Antibody recognizes both β-catenin and γ-catenin and demonstrates the specificity of γ-catenin expression silencing. The α-Tubulin (11H10) Rabbit mAb is used as a loading control.